Prostaglandins & Other Eicosanoids
Prostaglandins & Other Eicosanoids
Sensitive immunoassays for quantifying lipid mediators of inflammation — essential endpoints for evaluating irritation potential of personal care ingredients.
Lipid Mediators of Skin Irritation
Prostaglandins, leukotrienes, and thromboxanes are bioactive lipid mediators derived from arachidonic acid via the cyclooxygenase (COX) and lipoxygenase (LOX) pathways. These eicosanoids play central roles in skin irritation responses — mediating vasodilation, edema, pain, and inflammatory cell recruitment following exposure to irritant chemicals. Enzo Life Sciences provides a comprehensive portfolio of competitive ELISA kits for quantifying individual eicosanoid species in cell culture supernatants, skin tissue homogenates, and reconstructed human skin models with picogram-level sensitivity.
Prostaglandin E2 (PGE2)
High-sensitivity competitive ELISA for PGE2 — the primary prostaglandin mediating pain, vasodilation, and inflammatory responses in irritated skin. Detect PGE2 in keratinocyte and fibroblast culture supernatants at concentrations as low as 8.26 pg/mL.
Leukotriene Panel
ELISA kits for LTB4, LTC4, LTD4, and LTE4 — lipoxygenase pathway products that mediate vascular permeability, neutrophil chemotaxis, and mucus secretion. Assess the contribution of the 5-LOX pathway to irritation responses in skin exposure models.
Thromboxane & PGF2α
Competitive immunoassays for thromboxane B2 (stable metabolite of TXA2) and PGF2α quantification. These COX-derived mediators contribute to vasoconstriction and smooth muscle contraction associated with severe irritation and corrosion responses.
COX-2 & LOX Activity
Enzyme activity assay kits for cyclooxygenase-2 (COX-2) and 5-/12-/15-lipoxygenase. Measure upstream enzyme induction to identify which eicosanoid biosynthetic pathways are activated by irritant substances — enabling mechanistic understanding of the irritation response.
Featured Products
Explore our most popular kits and reagents for this application area:
- Prostaglandin E2 ELISA Kit
- Leukotriene B4 ELISA Kit
- Leukotriene C4 ELISA Kit
- Thromboxane B2 ELISA Kit
- COX-2 Activity Assay Kit
Prostaglandins & Other Eicosanoids — By the Numbers
Quantify Inflammatory Lipid Mediators
Contact our team to discuss your testing requirements or request product recommendations for your specific application.