Irritation & Corrosion Testing

Validated in vitro assays for skin irritation and corrosion assessment — supporting OECD-compliant alternative testing strategies for personal care ingredients.

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In Vitro Skin Safety Assessment

Regulatory agencies worldwide are driving the adoption of non-animal testing methods for skin irritation and corrosion classification. Enzo Life Sciences provides validated cell viability assays, cytotoxicity detection kits, and inflammatory marker panels that enable personal care companies to assess ingredient safety using reconstructed human skin models and cell-based systems compliant with OECD Test Guidelines 439 (skin irritation) and 431 (skin corrosion).

Cell Viability & Cytotoxicity

Quantitative measurement of cell viability using MTT, LDH release, and ATP-based luminescence assays. These endpoints form the foundation of OECD-compliant skin irritation testing protocols using EpiDerm, EpiSkin, and SkinEthic reconstructed tissue models.

Viability

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Inflammatory Cytokine Detection

Sensitive ELISA-based detection of IL-1α, IL-6, IL-8, and TNF-α released from keratinocytes and reconstructed skin models following exposure to test substances. These inflammatory mediators serve as mechanistic markers of irritation potential.

Cytokines

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Barrier Integrity Markers

Assays for tight junction proteins, transepithelial electrical resistance (TEER) correlates, and lipid peroxidation markers that indicate disruption of the skin barrier — a key early event in both irritation and corrosion.

Barrier

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Oxidative Stress Endpoints

Measure reactive oxygen species (ROS) generation, glutathione depletion, and 8-OHdG formation to evaluate oxidative damage caused by irritant substances. Our SENTINEL platform provides multi-parameter oxidative stress assessment.

Oxidative

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