Protocols
Protocols
Detailed, step-by-step experimental protocols developed and validated by our scientists. From ELISA procedures to HDAC activity assays, find the guidance you need for reliable, reproducible results.
Protocols by Technique
Find protocols organized by experimental method
ELISA Protocols
Sandwich ELISA, competitive ELISA, and multiplex procedures. Includes sample preparation, standard curves, incubation optimization, and troubleshooting guides.
Immunohistochemistry (IHC)
Tissue fixation, antigen retrieval, antibody staining, and detection protocols for paraffin-embedded and frozen tissue sections.
Western Blotting
Sample lysis, gel electrophoresis, membrane transfer, blocking, antibody incubation, and detection protocols optimized for Enzo antibodies.
Enzyme Activity Assays
FLUOR DE LYS HDAC, caspase, kinase, and other enzyme activity assay protocols. Includes plate reader setup and data analysis guidance.
Flow Cytometry
Cell surface and intracellular staining protocols, compensation setup, and gating strategies using Enzo fluorescent conjugates.
Cell-Based Assays
Cell viability, proliferation, apoptosis, and oxidative stress measurement protocols. Includes cell culture preparation and assay optimization tips.
Protocol Quality Standards
Most Downloaded Protocols
- Sandwich ELISA General Protocol — Complete guide for running any Enzo ELISA kit, including plate coating, sample preparation, and detection
- FLUOR DE LYS HDAC Activity Assay — Step-by-step fluorometric assay for Class I/II HDAC activity in cell lysates and purified enzymes
- Cortisol ELISA (ADI-900-071) — Optimized protocol for measuring cortisol in serum, plasma, saliva, and urine samples
- Oxidative Stress Panel — Comprehensive protocol for measuring total ROS, lipid peroxidation (TBARS), and total antioxidant capacity
- Immunofluorescence Staining — Detailed protocol for IF using Enzo primary antibodies with recommended secondary conjugates
- Caspase Activity Measurement — Fluorometric detection of caspase-3, -8, and -9 activity in apoptotic cell lysates
Need Help with a Protocol?
Our application scientists are available to assist with protocol optimization, troubleshooting, and custom application development.