Hit Validation
Hit Validation
Confirm and characterize screening hits with orthogonal assays, selectivity profiling, and mechanism-of-action studies to advance your most promising leads.
From Hits to Leads
Transitioning from primary screening hits to validated lead compounds requires rigorous confirmation using orthogonal assay formats. Enzo provides a comprehensive toolkit for dose-response characterization, counter-screening, selectivity profiling, and early ADMET assessment.
Hit Validation Workflow
Structured approach to hit confirmation and lead selection
Dose-Response Confirmation
Validated assay kits with Z’-factor data for precise IC50/EC50 determination across biochemical and cell-based formats.
Selectivity Profiling
Panel-based selectivity screening across related target families using enzymatic and binding assays.
Mechanism of Action
Cell-based assays for pathway analysis, target engagement confirmation, and phenotypic characterization.
Cytotoxicity & ADMET
Early toxicity assessment with cell viability, apoptosis, and metabolic activity assay panels.
Advance to Assay Development
Once you’ve confirmed your hits, our development team can help you translate validated assays into robust, scalable formats.