Home Basic Research Technology Platforms Tissue Analysis In situ Hybridization ISH AMPIVIEW® RNA probes

Powered by Enzo’s LoopRNA™ ISH Technology

AMPIVIEW® RNA probes are uniquely designed with the precision of targeted, sequence specific RNA probes, powered by Enzo’s LoopRNA™ ISH technology to deliver superior sensitivity. When AMPIVIEW® RNA probes hybridize to nucleic acid targets, loops form exposing biotin or digoxigenin labels, making these probes not only the most sensitive means of detection, but adaptable with existing immunohistochemistry (IHC) detection solutions and chromogens. Additionally, morphology of the sample is preserved and the signal of the targeted biomarker can be seen under the light microscope.

AMPIVIEW® RNA Probes Benefits

• High sensitivity and reliability
• Protocols for formalin-fixed, paraffin-embedded (FFPE) tissues and cells
• Mild protocols to preserve tissue morphology
• End-user can use existing ISH set ups and protocols
• Optimized with Enzo’s cost-effective IHC/ISH detection portfolio
• Flexible products ready for any workflow (Manual or Automated)
• Spatial Biology
• Physiological transcription analysis
• Disease model studies
• Biomarkers research
• Target identification and validation
• Early preclinical drug discovery analysis

AMPIVIEW® RNA Probes Benefits

Detection of HPV 16/18 in SiHa cells (1-3 HPV integrated copies) with A. AMPIVIEW™ HPV 16/18 sense RNA probes to detect viral DNA only and B. AMPIVIEW™ HPV 16/18 antisense RNA probes to detect both viral DNA and viral RNA in cells.

Applications

Background in the stroma shows non-specific binding and staining

AMPIVIEW® RNA Probes can Detect Copiesof DNA with Sense Probesand DNA and RNA with Antisense Probes

AMPIVIEW® Loop-RNA probes do not require lengthy protocol to aid the formation of signal structures required in branched DNA probe technology that in turn, tend to increase background.