Home Basic Research Technology Platforms Tissue Analysis Immuno histochemistry (IHC) Immunohistochemistry Detection Reagents

For decades, immunohistochemistry (IHC) has been an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. Enzo’s complete set of tools improve the quality and efficiency of your IHC process from start to finish. Avoid ambiguity with high-sensitivity and low background reagents that are compatible with manual and automated platforms. Combined with the market’s most extensive palette of unique chromogens, Enzo provides the flexibility needed for simple or complex protocols.

POLYVIEW® PLUS detection reagents are ready-to-use (RTU), biotin-free solutions developed by directly labeling immunoglobulins (anti-mouse or anti-rabbit) with a nanopolymer of alkaline phosphatase (AP) or horseradish peroxidase (HRP) using proprietary technology. This ensures consistent and reproducible immunodetection of primary antibodies (mouse or rabbit) or nucleic acid probes combined with label (digoxigenin or biotin) linker against nuclear, cytoplasmic and membrane antigens in different types of tissue specimens. The single-step IHC or two-step ISH methodology enables faster staining procedures with significantly lower background.

Key Features & Applications

• Biotin-free nanopolymer detection circumvents endogenous biotin background
• High intensity color development delivers sharp, crisp staining
• Compatible with both automated and manual platforms
• The Antibody Blocker/Diluent further reduces background (sold separately)
• Customize with HIGHDEF® chromogens of your choice (sold separately)
• Ready-to-use reagent
• Streptavidin-based detection with reduced carbohydrate and biotin background
• Suitable for both manual and automated platforms
• Streptavidin Blocker/Diluent further reduces background (sold separately)
• 5-10X more sensitive than avidin based detection
• Customize with HIGHDEF® chromogens of your choice (sold separately)
• Biotin-free nanopolymer-based system for superior multiplex antigen detection

POLYVIEW® PLUS

POLYVIEW® PLUS HRP (anti-mouse) reagent is suitable for use with mouse IgG antibodies.

SAVIEW® PLUS Reagents

POLYVIEW® PLUS AP (anti-rabbit) reagent is suitable for use with rabbit IgG antibodies.

MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green)

POLYVIEW® PLUS HRP (anti-rabbit) reagent is suitable for use with rabbit IgG antibodies.