Home Basic Research Technology Platforms Protein Analysis Western Blot

Western blotting is viewed as the gold standard for protein detection in molecular biology research. It is used to identify proteins within a cell or tissue lysate. Antibodies against your protein(s) of interest, bind to specific epitopes to identify the target protein within a lysate. Due to the high specificity of the binding, multiple target proteins can be identified on one membrane. Secondary antibodies then bind to your primary antibodies and when exposed to a substrate react, allowing for the visualization of the corresponding protein band.

Who wants to spend all that time in the dark?

Key Features & Applications

• High sensitivity – quickly visualize your bands with minimal overexposure risk and with no visible background.
• Convenient and easy to use – includes secondary antibody and all reagents necessary to visualize your bands.
• Specific – to bind your protein of interest or primary antibody
• Sensitive – for high detection and low background
• Reliable – for consistent results
• Validated – for Western blot application and stated species
• Check for equal transfer across membrane
• Ensure even loading between lanes
• Normalize and quantify your data
• Sensitive protein detection with low background and very low burn-through
• Provides high surface area for strong hydrophobic interactions (adsorb 50% more protein than nylon or nitrocellulose.)
• Broad solvent compatibility

Signal Develops In Front Of Your Eyes

Eventhough chemiluminescent detection is a common method of visualization this involves spending time in a tight dark room, with a timer and a list of exposure times, which are mostly chosen based on educated guesswork.

Primary Antibodies for Western Blotting

For high sensitivity, ease of use, and long-lasting signal, try our new WESTERNVIEW® Detection Kits

Western Blotting Membranes

PANC-1 cells treated with or without etoposide. Loaded total cell lysate (20 μg) and simultaneously incubated with tubulin and caspase antibodies before being developed with the WESTERNVIEW® Dual Detection Kit (Anti-Mouse / Anti-Rabbit).