ELISA kits with AMP’D® Technology
ELISA kits with AMP’D® Technology
Home Basic Research Technology Platforms Protein Analysis ELISA Immunoassays ELISA kits with AMP’D® Technology
Home Basic Research Technology Platforms Protein Analysis ELISA Immunoassays ELISA kits with AMP’D® Technology
Quantify Difficult-to-Detect Analytes
The AMP’D® ELISA Signal Amplification system is designed to replace traditional alkaline phosphatase (AP) substrates, such as pNPP (p-Nitrophenyl phosphate), with a combination substrate and amplifier system that results in greater sensitivity when compared to a traditional substrate ELISA.
Enzo’s AMP’D® Technology
In a conventional detection system, enzyme bound to the microtiter plate interacts directly with the substrate producing a color change where the resulting absorbance is directly proportional to the amount of captured analyte. In the Amp’d ELISA system, bound AP converts a substrate that is utilized in a second enzyme reaction system which is initiated by addition of the amplifier reagent. It is this amplification step that allows for greater (amplified) color production at lower analyte concentrations resulting in an increase in assay sensitivity.
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- Quantify difficult-to-detect analytes
- Minimal additional time needed to complete amplification step
- Flexible format for use with any immunometric, sandwich ELISAs where greater sensitivity is desired
- Convenient one or five 96-well plate sizes for high throughput analysis
- Easy-to-use, simple procedure with nominal ELISA protocol modification
AMP’D® ELISA Kits
In the Amp’d ELISA system, the critical substrate component, NADPH, is added to wells containing AP, and the AP reduces to NADH via release of a phosphate group. This reaction is allowed to proceed for an amount of time with the accumulated NADH being proportional to the amount of analyte/bound AP-conjugate. Upon the addition of the reconstituted amplifier reagent, this first reaction is quenched and the NADH feeds a second redox enzyme system. Here diaphorase utilizes NADH to reduce the iodonitrotetrazolium salt into formazan (purple color) producing NAD+. A counter enzymatic reaction then occurs where the NAD+ is reduced to NADH while ethanol is oxidized to acetaldehyde via alcohol dehydrogenase. These enzymatic reactions proceed for a period of time, recycling the NADH thus amplifying the original AP/substrate reaction. The resulting color intensity is ultimately proportional to the amount of bound analyte.
Our Featured Products
Our AMP’D® ELISA kit provides increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples
AMP’D® Certified kit and supporting reagents
Ultra-sensitive (7 pg/ml) AMP’D® HSP70 high sensitivity ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of human, mouse and rat Hsp70 (Hsp72), a major chaperone, cancer biomarker, and key cell stress regulator.
Explore ELISA kits with AMP'D® Technology - Enzo Solutions
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