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Detect Small And Large Analytes Accurately & Efficiently

Enzo offers hundreds of ELISA Assays in both immunometric and competitive assay formats. As scientists and manufacturers of kits, we understand the critical nature of your research.Each enzyme-linked immunosorbent assay is put through rigorous testing to ensure high precision, accuracy, sensitivity, and specificity. You can be confident that you will obtain reproducible results, day after day, and lot after lot.

Featured ELISA Kits

• Wells are washed to remove unbound molecules
• Add detection antibody and incubate to bind to antigen creating a “sandwich”
• Wash away excess detection antibody
• Add secondary antibody-enzyme conjugate and wash. Conjugated reporter enzyme is typically horseradish peroxidase (HRP) or alkaline phosphatase (AP)Note: This step may be omitted if the reporter enzyme is directly conjugated to the detection antibody
• Add substrate and incubate to develop signal
• Measure signal in a plate reader
• Sample is added to well coated with anti-species antibody
• Enzyme-conjugated antigen added to well with sample
• Capture antibody added to well and incubated. The antigen in the sample and the enzyme-conjugated antigen compete for binding to the capture antibody. The anti-species antibody coated in the well binds the capture antibody.
• Well is washed to remove excess antibody and enzyme-conjugated antigen
• Add substrate to develop signal
• Measure signal in plate reader

Why use our ELISA Kits?

OxytocinPGE2PEGylated proteinProtein ASerotoninTestosterone

Entrust your precious samples, time, and research budget to ELISA assay kits from Enzo

Enzyme-linked immunosorbent assay (ELISA) is an analytical, plate-based technique to detect and quantify the presence of a target analyte in a liquid sample using antibodies.

The Basics of ELISA Kits

Our ELISA Kits are validated in multiple sample matrices from serum and saliva to urine and feces