Home Basic Research Technology Platforms Genomic Analysis PCR Contamination Control of Genomic DNA

Contaminating genomic DNA (gDNA) in RNA preparations can lead to incorrect quantification of RNA in RT-PCR or high DNA background in RNA sequencing. This is particularly an issue during the quantification of low-copy transcripts or the sequencing of small RNA samples. Removal of gDNA is often a necessity to prevent high DNA background in RNA sequencing. This protocol can be applied to RNA preparations before next-generation sequencing (NGS).

The gDNA removal kit is based on a recombinant heat-labile dsDNase (HL-dsDNase) to remove gDNA from RNA preparations. Through HL-dsDNase technology, contaminating genomic DNA is cleaved at ambient temperature and the enzyme is irreversibly inactivated upon increasing the temperature over 50° C.

PCR Decontamination Kit

Minimizes pipetting steps and reduces hands-on time

PCR & Gel Clean-up Columns

Suitable for high-throughput experiments

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Eliminate gDNA contamination in RNA samples within 20 minutes