Home Basic Research Technology Platforms Genomic Analysis PCR AMPIPROBE® Nucleic Acid Detection

Enzo’s mission is to provide low-cost alternative solutions to the diagnostic market. Our AMPIPROBE® platform uses novel PCR design to enable the quantification of nucleic acids for clinically relevant targets. Incorporating our IP into the AMPIPROBE® assays allows Enzo to offer a low-cost option for nucleic acid detection in a price sensitive market.AMPIPROBE® assays are easily adaptable for laboratory use and cost-effective, without compromising on quality and performance. Compatible on open PCR platforms, AMPIPROBE® assays can be validated on existing instrumentation, eliminating the need for capital expenditures.

AMPIPROBE® is a proprietary technology that incorporates probe detection in primer design. It employs a combination of fluorescent reporter-labeled primers and quencher-labeled primers to amplify DNA, akin to traditional qPCR.

Key Features & Applications

• Clinical Specificity: 99.3%
• Size: 96 tests
• Adaptable to both automated open platforms (including GENFLEX®) and manual processing
• Sensitive quantitation of HCV viral loadLOD Serum = 5.5 IU/mL
• LOD Plasma = 7.9 IU/mL
• Low-cost alternative to other methods of viral load detection
• Compatible with most open qPCR platforms
• Complete set of controls including:High, Medium and Low quantitation controls
• Internal sample extraction control
• Negative sample control
• Smaller sample input allows remaining extracted samples to be used in other tests

Enzo’s AMPIPROBE® Technology

Once the signal decays to a defined threshold, a value is generated with respect to the corresponding cycle. The threshold cycle is indicative of the amount of target RNA or DNA in the sample.

AMPIPROBE® SARS-CoV-2 Test System (RUO)

The AMPIPROBE® HCV assay kit is Enzo’s landmark AMPIPROBE® assay for the quantitation of HCV viral load. It has been validated and approved by the New York State Department of Health to be used as a laboratory developed test to quantitatively measure HCV viral load.

AMPIPROBE® Women’s Health Pane

Our AMPIPROBE® SARS-CoV-2 Assay Kit (RUO) is a multiplexed assay designed for the detection of the SARS-CoV-2 virus. It contains two primer/probe sets specific to different SARS-CoV-2 genomic regions and primers/probes for internal control and negative control, human RNase P. This kit is intended to be used in combination with general RNA extraction kits, including AMPIXTRACT™ SARS-CoV-2 Extraction Kit (RUO) and generic qPCR instruments. This assay can also be used in manual settings or with open automated platforms such as the GENFLEX® Automated Instrument (please contact us for more information) or any other open platforms available on the market for the liquid handling process of RNA extraction and PCR set up.