Home Basic Research Technology Platforms Genomic Analysis Chromosomal Analysis BIOARRAY™ Amplification & Labeling Systems

Enzo’s BIOARRAY™ RNA Amplification & Labeling kits offer labeling solutions for biotin-based gene expression microarrays. Due to greater biotin incorporation and brighter signal, BIOARRAY™ RNA labeling is the best choice for routine GeneChip® analysis.

Biotin-labeled antisense RNA (aRNA) is generated from total cellular RNA samples in less than 24 hours. Incorporation of two biotin nucleotides yields brighter signal, improving data from microarray experiments.

Key Features & Applications

• Decrease experimental variation, and standardize data derived from microarrays.
• Maintain the value of legacy data by the continued use of the gold standard for GeneChip® visualization.
• Enables correlation of results from experiment-to-experiment, project-to-project and lab-to-lab.
• Production of large amounts of biotin-labeled RNA targets by in vitro transcription from bacteriophage T7 RNA polymerase promoters is available separately with our BioArray HighYield® RNA Transcript Labeling Kits.
• Rosen, MB et.al. Gene Expression Profiling in Wild-Type and PPARα-Null Mice Exposed to Perfluorooctane Sulfonate Reveals PPARα-Independent Effects. PPAR Research Volume 2010, Article ID 794739, doi:10.1155/2010/794739.
• Gabelt, BT et.al. Caldesmon effects on the actin cytoskeleton and cell adhesion in cultures HTM cells. Experimental Eye Research. Volume 82, issue 6, June 2006, 945-958.
• Generates sufficient aRNA for standard microarray analysis from as little as 20 ng of total input RNA.
• Entire amplification reaction can be performed in a single tube.
• Superior 3’/5’ transcript ratios demonstrating efficient in vitro transcription.
• Biotin-labeled aRNA can be purified using either magnetic beads or purification columns and reagents
• ENZ-42655-10 — 10 Reactions $1,071.00 BIOARRAY HIGHYIELD® RNA Transcript Labeling Kit (T7) quantity
• ENZ-42655-40 — 40 Reactions $3,444.00 BIOARRAY HIGHYIELD® RNA Transcript Labeling Kit (T7) quantity

BIOARRAY™ Single-round RNA amplification and biotin labeling system

Read the complete Application Note

Analyze Limiting Quantities of Total RNA

The kit is considered the gold standard end-labeling system for use with Affymetrix® DNA SNP (single nucleotide polymorphism), resequencing and prokaryotic microarrays. This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyze the addition of a single biotin-ddUMP (2’,3’-dideoxyuridine 5’-monophosphate) to the 3’-OH terminus of an amplified and fragmented target DNA molecule.

Change Country

Figure 1: Universal Human Reference RNA ranging from 20ng to 500ng was amplified in triplicate using BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System. The lowest amount of input (20ng) generated enough labeled aRNA for microarray analysis.