Home Basic Research Technology Platforms Cellular Analysis Antibodies Secondary Antibodies

Secondary antibodies can be monoclonal or polyclonal and are useful for detecting, sorting, and purifying antigens. A typical secondary antibody is a polyclonal antibody labelled with a probe which binds to a primary antibody which in turn has been bound to an antigen of interest. Because multiple secondary antibodies can bind to a single primary antibody it is possible to achieve increased sensitivity due to signal amplification.

Enzo Life Sciences offers secondary antibodies from a variety of species featuring conjugates appropriate for ELISA, WB, IHC, ICC, and FC.

Key Features & Applications

• Class/subclass of the primary antibody.
• Cross reactivity of the secondary.
• Preferred label based on downstream detection method.
• Western blot
• Immunohistochemistry
• Immunocytochemistry

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It is possible to create specificity to whole Immunoglobulins or smaller fragments depending on what is used to immunize the host animal and through downstream purifications. Most secondary antibodies are generated against a pool of IgG from the target species. This will allow binding to all Ig subclasses, heavy and light chains, fragments, and conserved domains. If a more specific target is desired, the host can be immunized with a specific target, for example IgG2 only. This will result in a secondary that only binds to species specific IgG2. Specificity can also be increased via column purification through a matrix to adsorb out cross-reacting antibodies.