Immunohistochemistry (IHC)
Immunohistochemistry (IHC) — Visualize Targets in Tissue
From primary antibodies to complete detection systems, Enzo Life Sciences provides IHC-validated reagents for crisp, specific staining on FFPE and frozen tissue sections.
Primary Antibodies
IHC-validated monoclonal and polyclonal antibodies targeting oncology markers, immune cell antigens, signaling proteins, and cytoskeletal components.
Secondary Antibodies
HRP- and AP-conjugated secondaries, biotinylated secondaries, and polymer-based detection reagents.
Detection & Chromogens
DAB, AEC, and alkaline phosphatase substrate systems for bright, permanent staining.
Antigen Retrieval
Citrate (pH 6.0) and EDTA (pH 9.0) buffers optimized for heat-induced epitope recovery on FFPE tissues.
Step 1: Fixation & Sectioning
Fix tissue in 10% NBF for 24–48 hours. Process for paraffin embedding. Cut 4–5 µm sections onto charged slides. For frozen, embed in OCT and snap-freeze.